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microarray hybridization solution version 2  (Danaher Inc)


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    Danaher Inc microarray hybridization solution version 2
    Microarray Hybridization Solution Version 2, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+solution+version+2/Microarray+Hybridization+Solution+v2%2E0/pmc01175033-123-5-10
    Average 93 stars, based on 116 article reviews
    microarray hybridization solution version 2 - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Microarray:

    Article Title: Integrated Metabolite and Transcript Profiling Identify a Biosynthetic Mechanism for Hispidol in Medicago truncatula Cell Cultures
    Article Snippet: The Amino Allyl cDNA Labeling Kit (Ambion) was used to label 25 μ g of total RNA following the manufacturer's protocol. .. The product was resuspended in 70 μ L of Microarray Hybridization Solution, version 2 (Amersham Biosciences), denatured at 95°C to 100°C for 2 min, pipetted onto the slides, and covered with a coverslip (Corning) before sealing in a hybridization chamber (Corning). ..

    Article Title: Oncogenic Property of Acrogranin in Human Uterine Leiomyosarcoma: Direct Evidence of Genetic Contribution inIn vivoTumorigenesis
    Article Snippet: .. Labeled probes were mixed with microarray hybridization solution version 2 (Amersham Biosciences) and formamide to yield a final concentration of 50%. ..

    Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens
    Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Microarray Hybridization Solution Version 2 (Amersham Biosciences) and formamide (Sigma Chemical Co, St Louis, MO, USA) to a final concentration of 50%. ..

    Article Title: Osteopontin is associated with nuclear factor kappaB gene expression during tail-suspension-induced bone loss.
    Article Snippet: Muneaki Ishijima, Yoichi Ezura⁎, Kunikazu Tsuji, Susan R. Rittling, Hisashi Kurosawa, David T. Denhardt, Mitsuru Emi, Akira Nifuji, Masaki Noda⁎ Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 3-10, Kanda-Surugadai 2-Chome, Chiyoda-Ku, Tokyo 101-0062, Japan Nelson Laboratories, Rutgers University, Piscataway, NJ 08854-8082, USA The Forsyth Institute, Boston, MA 02115, USA Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, Kawasaki, Japan Department of Orthopaedics, Juntendo University, School of Medicine, Tokyo 113-8421, Japan 21st Century Center of Excellence (COE) Program for Molecular Destruction and Reconstitution of Tooth and Bone, Tokyo Dental and Medical University, Japan Core to Core Program for Advanced Bone and Joint Science, Japan Society for the Promotion of Science, Tokyo Medical and Dental University, Japan Hard Tissue Genome Research Center, Tokyo Medical and Dental University

    Article Title: Tumor suppressor p16INK4a--modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells.
    Article Snippet: Its efficiency was routinely monitored by adsorption measurements using a Nanodrop ND100 UV ⁄VIS spectrophotometer. .. Aliquots containing the labeled cDNA were diluted to a volume of 200 lL in Microarray Hybridization Solution, Version 2 (Amersham Biosciences, Braunschweig, Germany) and hybridized onto the GlycoProfiler microarray (Scienion, Berlin, Germany) using an a-Hyb hybridization station (Miltenyi, Cologne, Germany) for 16 h at 42 C. Subsequent washing steps were then performed using 2 · NaCl ⁄ Cit, 0.2% SDS as first, 1 · NaCl ⁄Cit as second and 0.05 · NaCl ⁄Cit as third wash buffer. .. The glass slides were dried in the centrifuge and scanned using the Agilent DNA Microarray Scanner, and the obtained data were subjected to analysis using the feature extraction 8.0 software (Agilent).

    Article Title: Identification of histological markers for malignant glioma by genome-wide expression analysis: dynein, alpha-PIX and sorcin.
    Article Snippet: Glioblastoma multiforme (GBM), the most malignant class of glial neoplasm (grade IV in WHO criteria), carries the worst clinical prognosis among primary brain tumors in adults.. To identify a set of genes involved in the tumorigenesis of GBM, we evaluated expression profiles of GBM tissues from 11 patients using a cDNA microarray representing 25,344 human genes.. By comparing the profiles with those of normal brain tissue, we identified a number of differentially expressed genes: 54 with increased expression and 45 with reduced expression in GBMs.

    Hybridization:

    Article Title: Integrated Metabolite and Transcript Profiling Identify a Biosynthetic Mechanism for Hispidol in Medicago truncatula Cell Cultures
    Article Snippet: The Amino Allyl cDNA Labeling Kit (Ambion) was used to label 25 μ g of total RNA following the manufacturer's protocol. .. The product was resuspended in 70 μ L of Microarray Hybridization Solution, version 2 (Amersham Biosciences), denatured at 95°C to 100°C for 2 min, pipetted onto the slides, and covered with a coverslip (Corning) before sealing in a hybridization chamber (Corning). ..

    Article Title: Oncogenic Property of Acrogranin in Human Uterine Leiomyosarcoma: Direct Evidence of Genetic Contribution inIn vivoTumorigenesis
    Article Snippet: .. Labeled probes were mixed with microarray hybridization solution version 2 (Amersham Biosciences) and formamide to yield a final concentration of 50%. ..

    Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens
    Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Microarray Hybridization Solution Version 2 (Amersham Biosciences) and formamide (Sigma Chemical Co, St Louis, MO, USA) to a final concentration of 50%. ..

    Article Title: Osteopontin is associated with nuclear factor kappaB gene expression during tail-suspension-induced bone loss.
    Article Snippet: Muneaki Ishijima, Yoichi Ezura⁎, Kunikazu Tsuji, Susan R. Rittling, Hisashi Kurosawa, David T. Denhardt, Mitsuru Emi, Akira Nifuji, Masaki Noda⁎ Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 3-10, Kanda-Surugadai 2-Chome, Chiyoda-Ku, Tokyo 101-0062, Japan Nelson Laboratories, Rutgers University, Piscataway, NJ 08854-8082, USA The Forsyth Institute, Boston, MA 02115, USA Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, Kawasaki, Japan Department of Orthopaedics, Juntendo University, School of Medicine, Tokyo 113-8421, Japan 21st Century Center of Excellence (COE) Program for Molecular Destruction and Reconstitution of Tooth and Bone, Tokyo Dental and Medical University, Japan Core to Core Program for Advanced Bone and Joint Science, Japan Society for the Promotion of Science, Tokyo Medical and Dental University, Japan Hard Tissue Genome Research Center, Tokyo Medical and Dental University

    Article Title: Tumor suppressor p16INK4a--modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells.
    Article Snippet: Its efficiency was routinely monitored by adsorption measurements using a Nanodrop ND100 UV ⁄VIS spectrophotometer. .. Aliquots containing the labeled cDNA were diluted to a volume of 200 lL in Microarray Hybridization Solution, Version 2 (Amersham Biosciences, Braunschweig, Germany) and hybridized onto the GlycoProfiler microarray (Scienion, Berlin, Germany) using an a-Hyb hybridization station (Miltenyi, Cologne, Germany) for 16 h at 42 C. Subsequent washing steps were then performed using 2 · NaCl ⁄ Cit, 0.2% SDS as first, 1 · NaCl ⁄Cit as second and 0.05 · NaCl ⁄Cit as third wash buffer. .. The glass slides were dried in the centrifuge and scanned using the Agilent DNA Microarray Scanner, and the obtained data were subjected to analysis using the feature extraction 8.0 software (Agilent).

    Article Title: Identification of histological markers for malignant glioma by genome-wide expression analysis: dynein, alpha-PIX and sorcin.
    Article Snippet: Glioblastoma multiforme (GBM), the most malignant class of glial neoplasm (grade IV in WHO criteria), carries the worst clinical prognosis among primary brain tumors in adults.. To identify a set of genes involved in the tumorigenesis of GBM, we evaluated expression profiles of GBM tissues from 11 patients using a cDNA microarray representing 25,344 human genes.. By comparing the profiles with those of normal brain tissue, we identified a number of differentially expressed genes: 54 with increased expression and 45 with reduced expression in GBMs.

    Labeling:

    Article Title: Oncogenic Property of Acrogranin in Human Uterine Leiomyosarcoma: Direct Evidence of Genetic Contribution inIn vivoTumorigenesis
    Article Snippet: .. Labeled probes were mixed with microarray hybridization solution version 2 (Amersham Biosciences) and formamide to yield a final concentration of 50%. ..

    Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens
    Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Microarray Hybridization Solution Version 2 (Amersham Biosciences) and formamide (Sigma Chemical Co, St Louis, MO, USA) to a final concentration of 50%. ..

    Article Title: Osteopontin is associated with nuclear factor kappaB gene expression during tail-suspension-induced bone loss.
    Article Snippet: Muneaki Ishijima, Yoichi Ezura⁎, Kunikazu Tsuji, Susan R. Rittling, Hisashi Kurosawa, David T. Denhardt, Mitsuru Emi, Akira Nifuji, Masaki Noda⁎ Department of Molecular Pharmacology, Medical Research Institute, Tokyo Medical and Dental University, 3-10, Kanda-Surugadai 2-Chome, Chiyoda-Ku, Tokyo 101-0062, Japan Nelson Laboratories, Rutgers University, Piscataway, NJ 08854-8082, USA The Forsyth Institute, Boston, MA 02115, USA Department of Molecular Biology, Institute of Gerontology, Nippon Medical School, Kawasaki, Japan Department of Orthopaedics, Juntendo University, School of Medicine, Tokyo 113-8421, Japan 21st Century Center of Excellence (COE) Program for Molecular Destruction and Reconstitution of Tooth and Bone, Tokyo Dental and Medical University, Japan Core to Core Program for Advanced Bone and Joint Science, Japan Society for the Promotion of Science, Tokyo Medical and Dental University, Japan Hard Tissue Genome Research Center, Tokyo Medical and Dental University

    Article Title: Tumor suppressor p16INK4a--modulator of glycomic profile and galectin-1 expression to increase susceptibility to carbohydrate-dependent induction of anoikis in pancreatic carcinoma cells.
    Article Snippet: Its efficiency was routinely monitored by adsorption measurements using a Nanodrop ND100 UV ⁄VIS spectrophotometer. .. Aliquots containing the labeled cDNA were diluted to a volume of 200 lL in Microarray Hybridization Solution, Version 2 (Amersham Biosciences, Braunschweig, Germany) and hybridized onto the GlycoProfiler microarray (Scienion, Berlin, Germany) using an a-Hyb hybridization station (Miltenyi, Cologne, Germany) for 16 h at 42 C. Subsequent washing steps were then performed using 2 · NaCl ⁄ Cit, 0.2% SDS as first, 1 · NaCl ⁄Cit as second and 0.05 · NaCl ⁄Cit as third wash buffer. .. The glass slides were dried in the centrifuge and scanned using the Agilent DNA Microarray Scanner, and the obtained data were subjected to analysis using the feature extraction 8.0 software (Agilent).

    Article Title: Identification of histological markers for malignant glioma by genome-wide expression analysis: dynein, alpha-PIX and sorcin.
    Article Snippet: Glioblastoma multiforme (GBM), the most malignant class of glial neoplasm (grade IV in WHO criteria), carries the worst clinical prognosis among primary brain tumors in adults.. To identify a set of genes involved in the tumorigenesis of GBM, we evaluated expression profiles of GBM tissues from 11 patients using a cDNA microarray representing 25,344 human genes.. By comparing the profiles with those of normal brain tissue, we identified a number of differentially expressed genes: 54 with increased expression and 45 with reduced expression in GBMs.

    Concentration Assay:

    Article Title: Oncogenic Property of Acrogranin in Human Uterine Leiomyosarcoma: Direct Evidence of Genetic Contribution inIn vivoTumorigenesis
    Article Snippet: .. Labeled probes were mixed with microarray hybridization solution version 2 (Amersham Biosciences) and formamide to yield a final concentration of 50%. ..

    Article Title: Characterization of histopathology and gene-expression profiles of synovitis in early rheumatoid arthritis using targeted biopsy specimens
    Article Snippet: The PCR products were spotted in duplicate on type VII glass slides (Amersham Biosciences) with a Microarray Spotter Generation III (Amersham Biosciences). .. Labeled probes were mixed with Microarray Hybridization Solution Version 2 (Amersham Biosciences) and formamide (Sigma Chemical Co, St Louis, MO, USA) to a final concentration of 50%. ..

    Article Title: Identification of histological markers for malignant glioma by genome-wide expression analysis: dynein, alpha-PIX and sorcin.
    Article Snippet: Glioblastoma multiforme (GBM), the most malignant class of glial neoplasm (grade IV in WHO criteria), carries the worst clinical prognosis among primary brain tumors in adults.. To identify a set of genes involved in the tumorigenesis of GBM, we evaluated expression profiles of GBM tissues from 11 patients using a cDNA microarray representing 25,344 human genes.. By comparing the profiles with those of normal brain tissue, we identified a number of differentially expressed genes: 54 with increased expression and 45 with reduced expression in GBMs.



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